BioScience2005 - from genes to systems
17-25 July 2005
Glasgow, UK

Science Programme

 

Sunday 17 July
16:00-17:00
Opening Plenary Lecture
Robert J. Lefkowitz
17:00-18:00
Plenary Lecture
Matthias Mann

Mini Symposia:

Monday 18 July Tuesday 19 July Wednesday 20 July Thursday 21 July
MORNING
8:30-9:30
Plenary Lecture
Wolfgang Baumeister
8:30-9:30
Novartis Medal Lecture
Alan Hall
8:30-9:30
Plenary Lecture
Walter Kolch
8:30-9:30
The Colworth Medal Lecture
Ian Collinson
Nuclear organization and gene expression Mechanisms of crossing-over: genetic and biochemical approaches   Mechanisms of translation
The keys to actin: regulators of the Arp2/3 complex ARF GTPases: integrators of lipid signalling, membrane traffic and action Protein phosphorylation in the control of synaptic vesicle dynamics Regulation of signalling by cellular location and sequestration
    Lysophosphatidic acid and sphingosine 1-phosphate signalling Role of thiol redox changes in proteins involved in signalling and oxidative damage
Genetic control of plant morphology - in the laboratory during domestication and in evolution Stem cells in development and disease Chromatin in development and disease  
Lipid - protein interactions: mechanistic and functional studies Coordination of clocks with the environment Evolutionary diversity of organellar metabolism Mechanisms of ABC transporters
Systematic functional analyses of genomes Metabolomics: the link between genomics, proteomics and phenotype Obesity, appetite and adipokines Amyloidogenic proteins and their therapeutic implications
LUNCH
12:45-13:45
Stratagene Workshop
A systems biology approach to research - using Interaction ExplorerT Software to bring biological context to your gene/protein lists.
12:45-13:45
Miltenyi Biotec Workshop
Easier done than said - Explore the experimental power of MACSmolecular Research Systems
   
  Promega UK Young Biochemist 2005    
AFTERNOON
14:00-15:00
Plenary Lecture
P. Leslie Dutton
Sponsored by 'TiBS'
14:00-15:00
Plenary Lecture
David Stuart
14:00-15:00
The Keilin Memorial Lecture
Martin Brand
13:30-14:30
The Jubilee Lecture
Richard Jackson
Nuclear organization and gene expression Topoisomerases and helicases: molecular motors on DNA    
Emerging cellular roles for actin polymerization ARF GTPases integrators of lipid signalling, membrane traffic and actin Mechanisms of synapse assembly and plasticity  
  New frontiers of cAMP signalling Lysophosphatidic acid and sphingosine 1-phosphate signalling Nitric oxide signalling - new targets, new biochemistry
Developmental mechanisms Stem cells in development and disease Translational control in development  
Lipid - protein interactions mechanistic and functional studies Conservation and convergence of clock elements Biochemistry and molecular biology of variable antigenic surface proteins of parasites Molecular aspects of protein translocation
Systematic functional analyses of genomes Metabolomics: the link between genomics, proteomics and phenotype    
  Insulin and leptin signalling in cognition and plasticity Obesity, appetite and adipokines Amyloidogenic proteins and their therapeutic implications
  Mechanisms of gene regulation Free radicals and cyclic nucleotide signalling pathways: nitric oxide, reactive oxygen species, cAMP and cGMP  
Making biosciences teaching FUN!      


Keynote Speakers

Sunday 17 July 2005 16:00-17:00
Robert J. Lefkowitz  (HHMI, MD, USA)
Transduction of Receptor Signals by b-arrestins
Chair: Miles Houslay (Glasgow, UK)
Sunday 17 July 2005 17:00-18:00
Matthias Mann  (Odense, Denmark) The EMBO Plenary Lecture
Quantitative proteomics as a basis for systems biology
Chair: Jean Thomas (Cambridge, UK)
Monday 18 July 2005 08:30-09:30
Wolfgang Baumeister  (Martinsried, Germany)
Mapping molecular landscapes inside cells by cryoelectron tomography
Chair: Chris Leaver (Oxford, UK)
Monday 18 July 2005 14:00-15:00
P. Leslie Dutton  (Pennsylvania, PA, USA) Sponsored by 'Trends in Biochemical Sciences'
Darwin at the molecular scale: selection and variance in electron transfer proteins
Chair: Peter Rich (University College London, UK)
Tuesday 19 July 2005 08:30-09:30
Alan Hall  (University College London, UK) The Novartis Medal Lecture
Rho GTPases in cell biology
Chair: Anne Ridley (Ludwig Institute, London, UK)
Tuesday 19 July 2005 14:00-15:00
David Stuart  (Oxford, UK)
Lessons from Nature�s pirates: the complex machinery of viruses
Chair: Martin Humphries (Manchester, UK)
Wednesday 20 July 2005 08:30-09:30
Walter Kolch  (Beatson Institute, Glasgow, UK)
The Raf kinase signalling pathway
Chair: Melanie Welham (Bath, UK)
Wednesday 20 July 2005 14:00-15:00
Martin Brand  (Cambridge, UK) The Keilin Memorial Lecture
The efficiency and plasticity of mitochondrial energy transduction
Chair: Brian Beechey (Liverpool, UK)
Thursday 21 July 2005 08:30-09:30
Ian Collinson  (Bristol, UK) The Colworth Medal Lecture
Atomic model of the membrane bound protein translocation complex SecYEG
Chair: Shamshad Cockcroft (University College London, UK)
Thursday 21 July 2005 13:30-14:30
Richard Jackson  (Cambridge, UK) The Jubilee Lecture
Alternative mechanisms of initiating translation of mammalian mRNAs
Chair: Sarah Newbury (Newcastle-upon-Tyne, UK)


Programme Committee

Chair: Keith Gull (Oxford, UK)
Brian Beechey (Liverpool, UK)
John Coggins (Glasgow, UK)
Jim Naismith (St Andrews, UK)
Mary Sugden (QM London, UK)
Kevin Brindle (Cambridge, UK)
David Rice (Sheffield, UK)
David Barford (ICR London, UK)
Neil Bruce (York, UK)
Anthony Magee (Imperial London, UK)


Translation UK (Satellite Meeting to BioScience2005)

Western Infirmary, Glasgow, UK
21-23 July 2005


Translation UK will bring together experts in the field to discuss the latest developments in research of all aspects of gene expression control at the levels of protein synthesis and mRNA turnover.

Each session will feature oral presentations from invited international speakers and those selected from abstracts submitted. A poster presentation session will provide an opportunity for delegates to present work that cannot be accommodated within the oral programme.

The oral sessions will feature presentations on ribosome and translation factor structure and interactions, translation mechanisms, regulation of translation factors, translational control in development and disease, and control of translation by mRNA turnover.

Translation UK will be a 'satellite meeting' to immediately follow the Biochemical Society's annual flagship meeting - BioScience2005. BioScience2005 will feature two additional sessions on translation and its control and will include the presentation of the Jubilee Lecture by Richard Jackson (Cambridge, UK).

Proceedings of invited speakers will be published in Biochemical Society Transactions

 

Abstracts

Nuclear organization and gene expression

Abstracts 0300–0317
Monday 18 July 18:00–19:30
0300 Role of 3C proteinase in human parechoviruses infections
D.R.F. Ghazi, A.A. Gorzin, B. Dabirmanesh and D.R.G. Stanway
0301 Search for molecular markers associated with resistance to Schistosoma mansoni infection in the snail Biomphalaria glabrata under laboratory conditions
S. Hamdy and I. Haggag
0302 Poster withdrawn
0303 Adrenomedullin receptor (CLR/RAMP2) signalling in human microvascular endothelial cells
N. Schwarz, S. Kapas and J.P. Hinson
0304 Tumor suppressor menin-mediated transcription of caspase 8: its role in suppression of inherited endocrine tumorigenesis
P. La, A.C. Silva, C. Krankel and X. Hua
0305 Polycistronic and Pol I mediated transcription of protein coding genes in trypanosomes: an in silico analysis of RNA polymerases
S. Kelly, B. Wickstead and K. Gull
0306 Coexpression of CYP1A1 and hTERT in lung cancer cell lines
K.K. Mohammed, R.W. Lea and A. Shervington
0307 Cholesterol linked to chlorambucil and one of its nitro-derivatives via an ester bridge as possible candidates for the treatment of glioma
L.A. Shervington, N. Cruickshanks and A.A. Shervington
0308 Can MGMT expression be used as a marker for treatment in glioma?
R. Patel, R. Lea and A. Shervington
0309 Hsp90 and oestrogen receptor role in glioma
N.A. Cruickshanks, L.A. Shervington, R.W. Lea, G.A. Roberts, R. Atkinson-Dell and A.A. Shervington
0310 MNK1/2-mediated phosphorylation results in cytoplasmic accumulation and recruitment of hnRNP A1 to cytoplasmic stress granules
S. Guil, J.C. Long and J.F. Caceres
0311 Chromosome positioning in porcine differentiation
H.A. Foster and J.M. Bridger
0312 Development of a potato microarray for food safety analysis
K. Cankar, J. van Dijk, K. Gruden, L. Shepherd, D. Stewart, C. Leifert, H. Davies and E. Kok
0313 Accumulation of g-globin mRNA in human erythroid cells treated with psoralens
I. Lampronti, N. Bianchi, M. Borgatti, R. Piva, M. Zennaro and R. Gambari
0314 Induction of apoptosis of human osteoclasts by a decoy molecule against a negative regulator of estrogen receptor alpha transcription
L. Penolazzi, E. Lambertini, M. Zennaro, M. Borgatti, N. Bianchi, I. Lampronti, R. Gambari and R. Piva
0315 ’Structural maintenance of chromosomes’ proteins in an early branching eukaryote
E. Gluenz, B. Wickstead and K. Gull
0316 Genome organisation in nuclear disease
J.M. Bridger, I.R. Kill and K.J. Meaburn
0317 Transcriptional control by chromosome-associated protein phosphatase 1
D. Bennett, A. Rudenko, P. Glenday, E. Lyulcheva, S. Tan and L. Alphey

The keys to actin: regulators of the Arp2/3 complex

Abstracts 0318–0319, 0556
Monday 18 July 18:00–19:30
0318 Regulation of actin-based protrusions by the SCAR complex
N. Ibarra, S. Blagg and R.H. Insall
0319 Involvement of the RASSF1A tumour suppressor gene in controlling cell migration
A. Dallol, A. Agathanggelou, S. Tommasi, G.P. Pfeifer, E.R. Mahe and F. Latif

Genetic control of plant morphology - in the laboratory, during domestication and in evolution

Abstract 0320
Monday 18 July 18:00–19:30
0320 Transgenic analysis of two proteins of RecA homologs, RAD51 and RAD51C, reveals that RAD51 is crucial in DNA repair in Arabidopsis
M.-Y. Jang, S.-G. Kim and J.-S. Kim

Mechanisms of crossing-over: genetic and biochemical approaches

Abstracts 0321, 0557
Tuesday 19 July 18:00–19:30
0321 Properties of RadB, a Rad51 paralogue in archaea
C.P. Guy, A.A. Brindley, M.J. Warren and E.L. Bolt

ARF GTPases: integrators of lipid signalling, membrane traffic and actin

Abstracts 0322–0325, 0558–0561
Tuesday 19 July 18:00–19:30
0322 Intracellular traffic of secretory organelles in ECL cells of stomach
C.-M. Zhao, R. Håkanson and D. Chen
0323 A putative bifunctional Plasmodium falciparum guanylyl cyclase-activating protein (PfGCAP) exhibits ARF-induced phosphatidylinositol 4-phosphate 5-kinase activity
W. Leber, A. Skippen, W. Deng, S. Cockcroft, D.A. Baker
0324 Centaurin b4 Arf-GAP in melanoma
R. Martin and T. Jackson
0325 Involvement of "lipid rafts" in the binding of some enteroviruses
A.B.S. Khojine and D.J. Evans

Stem cells in development and disease

Abstracts 0326–0341, 0562
Tuesday 19 July 18:00–19:30
0326 In vitro transdifferentiation of hepatoma cells into functional pancreatic cells
W.-C. Li, D. Tosh and J.M.W. Slack
0327 Reverse transfection for human embryonic stem cell studies
A.E. Topolska, M. Dottori, P. Jamshidi and M.F. Pera
0328 Effect of BCR/ABL tyrosine kinase in haemopoietic progenitor cell motility and PIP3 generation in response to SDF-1a
H.G. Hamzah, Y. Lu, A. Pierce, A. Gray, C.P. Downes and A.D. Whetton
0329 Mesenchymal stem cells and multi-potent adult progenitor cells are derived from the same precursor population in human bone marrow
D. Craeye, R. Raedt, B. Vandekerckhove, J. Plum, K. Gevaert, J. Vandekerckhove and J.A. Pinxteren
0330 Flk-1 signals involved in embryonic stem cell differentiation.
L.A. McRae and M.J. Welham
0331 Genetic analyses of phosphoinositide 3-kinase (PI3K)–dependent signalling during murine ES cell self-renewal and differentiation
M.P. Storm, H.K. Bone and M.J. Welham
0332 Comparative proteome analysis of the human foetal pancreas
C. Wong, L. Ruban, N. Jenkins and H. Moore
0333 Analysis of palmitic acid or pro-inflammatory cytokine induced changes in the expression of NADPH oxidase component p47-phox in the BRIN BD11 clonal pancreatic cell line
D. Keane, G. Cunningham and P. Newsholme
0334 The receiving of initial cultures of embryonic stem cells from mice embryos by use of cytokine leukemia inhibitory factor (LIF).
I.V. Kapralova and L.M. Mezhevikina
0335 Activin/nodal signals involved in propagation of mouse ES cells
K. Ogawa, A. Saito, H. Matsui, H. Suzuki, S. Ohtsuka, D. Shimosato, Y. Morishita, T. Watabe, K. Miyazono and H. Niwa
0336 Expression of non-neuronal cholinergic system in human epithelial cells and its role in cancer.
A. Pons-Castillo, L. Galbis-Martinez, S. Nieto-Ceron, P. Martinez-Hernandez, J.C. Morote-Garcia, C.J. Vidal and J. Cabezas-Herrera
0337 Investigation of the in vivo consequences of over-expression of the pluripotency determinant Nanog
A. Yates, J. Nichols, A. Smith and I. Chambers
0338 Poster withdrawn
M.A. Hornsey, D. Tosh and J.M.W. Slack
0339 Barrett’s metaplasia – a developmental switch?
J.M. Quinlan, J.M. Farrant, D. Tosh and J.M.W. Slack
0340 Effects of Achillea millefolium L. extract on testicular development in immature Balb/C mice.
K. Parivar and P. Kerishchi
0341 Haemostatic factors in mouse embryonic stem cell differentiation
W.S. Gilmore, R.W. Freeburn and H. Weadon

Molecular clocks (1): coordination of clocks with the environment

Abstracts 0342–0345
Tuesday 19 July 18:00–19:30
0342 The tango gene regulates circadian light responses in Drosophila melanogaster
L.G.S.R. Bauzer, Ö. Özkaya-Rosato, V. Codd and E. Rosato
0343 Organ specificity in the circadian control of plant gene expression
S. Sullivan, M. Shenton and H.G. Nimmo
0344 Identification of calcium-related circadian signalling components in Arabidopsis thaliana using a targetted screen
K.E. Hubbard, C.T. Hotta, J.M. Davies and A.A.R. Webb
0345 Dissecting morning specific transcription of the Arabidopsis clock gene LHY
J.-Y. Kim, M. Spensley, B. Taylor and I. Carré

Metabolomics: the link between genomics, proteomics and phenotype

Abstracts 0346–0352, 0564–0565
Tuesday 19 July 18:00–19:30
0346 Thermal inactivation of alkali stable xylanases (XylA and XylB) from alkali tolerant fungus Aspergillus fischeri Fxn 1: effect of trehalose on their thermostability
S.S. Rajan, A. Balasubramaniem, C. Krishnan, M. Dempsey and G. Paramasamy
0347 In Saccharomyces cerevisiae impaired PRPP synthesis causes valproate sensitivity
S. Vavassori, L.M. Schweizer and M. Schweizer
0348 Revelation of the complexity of gastric acid regulatory mechanism by genetic dissection
D. Chen, L. Friis-Hansen, R. Håkanson and C.-M. Zhao
0349 Development of an aptamer-based biochip assay for E-selectin
P. O’Hagan, V. Hodges, S.P. Fitzgerald and R.J.H. Davies
0350 Metabolic flux distribution analysis by 13C tracer experiments through Markov Chain Monte Carlo method
J. Yang, S. Wongsa, V. Kadirkamanathan, S.A. Billings and P.C. Wright
0351 Localization of a Fusarium-induced stress associated protein in wheat (Triticum aestivum) root
B. Mittra, J. Mishra, S.L. Henry, T.K. Das and P. Mohanty
0352 Using Aspergillus niger metabolic engine in Aspergillus terreus
G. Tev?z, M. Ben?cina, A. Kern, A. Glieder, M. Legi?sa

Protein phosphorylation in the control of synaptic vesicle dynamics

Abstracts 0353–0356
Wednesday 20 July 15:30–16:30
0353 Calcium-dependent interactions in synaptic vesicle endocytosis
H.L. Falconer and M.A. Cousin
0354 Developmental change in the calcium sensor for synaptic vesicle endocytosis in central nerve terminals
K.J. Smillie, G.J.O. Evans and M.A. Cousin
0355 CLIC proteins interact with dynamin I in nerve terminals and have specific subcellular localisation in neurons
H. Singh, M.A. Cousin and R.H. Ashley
0356 Akt/PKB in regulated exocytosis
G. Prescott, G. Evans, J. Barclay, S.-R. Jo, R. Burgoyne, M. Birnbaum and A. Morgan

Lysophosphatidic acid and sphingosine 1-phosphate signalling

Abstracts 0357–0362
Wednesday 20 July 15:30–16:30
0357 Mammalian phospholipase D1 and D2 are caspase-3 substrates
M.N. Hodgkin, M.H. Wright and M.J. Farquhar
0358 Identification of caspase-3 mediated cleavage sites in PLD1b
M.H. Wright, M.J. Farquhar and M.N. Hodgkin
0359 A potential role of sphingosine-1-phosphate in maintaining human embryonic stem cells in culture
K. Inniss, L. Ruban, N. Jenkins and H. Moore
0360 p85 beta PIX differentially regulates cell motility via FAK and p38 MAP kinase phosphorylations
C.G. Park and H.Y. Lee
0361 Autotaxin: regulation of activity and role in development
L.A. van Meeteren, P. Ruurs, P. Bouwman, J. Jonkers and W.H. Moolenaar
0362 Intracellular signalling pathways underlying sphingosine-1-phosphate-induced constriction of human blood vessels
N.K. Hudson, M. Wareing, M. O’Hara, D.G. Hemmings, D. Halliday, P.N. Baker and M.J. Taggart

Chromatin in development and disease

Abstracts 0363–0365, 0566–0567
Wednesday 20 July 15:30–16:30
0363 Molecular studies on relationship between diabetes mellitus and viral hepatitis B and C
S. Ali, N. Ahmad, M. Asghar, N. Badar and J.A. Qureshi
0364 Identification of transcriptional activation and repression domains in the DNA damage response protein TopBP1
R.H.G. Wright and I.M. Morgan
0365 Development of a yeast-based assay system for monitoring microsatellite instability
A. Marden, R. Walmsley, L. Schweizer and M. Schweizer

Evolutionary diversity of organellar metabolism

Abstracts 0366–0372
Wednesday 20 July 15:30–16:30
0366 Autophagy and related processes in trypanosomatids: insights from genomic and bioinformatics analyses
M. Herman, S. Gillies, P.A. Michels and D.J. Rigden
0367 The role of thiol-dependant reductase 1 (TDR1) in Leishmania
J.C. McGregor, H. Denton and G.H. Coombs
0368 Golgi-type maturation of carbohydrate chains of glycoproteins occurs in the ER of brain neurons
E. Vermeulen, C. Parkyn, A. Warley, M. Perkington, A. Jen and R. Morris
0369 The lipoic acid salvage pathway of Plasmodium falciparum
S. Günther and S. Müller
0370 The a-keto acid dehydrogenase complexes of the malaria parasite Plasmodium falciparum
P.J. McMillan and S. Müller
0371 Post-genomic views of a "unique" metabolism in the eukaryotic flagellum
M.L. Ginger
0372 Lipoic acid: synthesis and ligation in Plasmodium falciparum
L.J.M. Wallace, M.S. Günther and S. Müller

Mechanisms of synapse assembly and plasticity

Abstracts 0373–0377, 0568
Wednesday 20 July 15:30–16:30
0373 Neuromuscular protection from organophosphate poisoning using plant-derived recombinant human AChE-R
T. Evron, B.C. Geyer, S.P. Fletcher, M. Muralidharan, E. Podoly, L. BenMoyal-Segal, H. Soreq and T.S. Mor
0374 Lipid rafts, palmitoylation and exocytosis
C. Salaün, G.W. Gould and L.H. Chamberlain
0375 Thy-1 interacts with aVb3 integrin in astrocytes and triggers bidirectional signals in vitro
T. Hermosilla, E. Diaz, A.F.G. Quest and L. Leyton
0376 Integrin modulation of synaptic efficacy
L.A. Cingolani, L.M.Y. Yu, T. Branco and Y. Goda
0377 A proposed mechanism for achieving nitrogen balance in glutamate–glutamine neurotransmitter cycling
P.K. Maciejewski and D.L. Rothman

Translational control in development

Abstracts 0378, 0569–0570
Wednesday 20 July 15:30–16:30
0378 Post-transcriptional regulation in Xenopus embryos: role and targets of EDEN-BP
H.B. Osborne, C. Gautier-Courteille, A. Graindorge, Y. Audic, R. Thuret, N. Pollet and L. Paillard

Mechanisms of translation

Abstracts 0379–0390, 0571–0572
Wednesday 20 July 15:30–16:30
0379 The interplay of initiation factors in ribosomal subunit association
A. Antoun, M. Pavlov and M. Ehrenberg
0380 Identification of the trans-acting factors of the L- and N-myc IRES
L. Cobbold, K. Spriggs, C.L. Joplin and A.E. Willis
0381 Investigating the subcellular localisation of translation initiation factor eIF4F in NIH3T3 fibroblasts
M. Willett and J. Pain
0382 Trans-acting factor requirement in IRES activation during apoptosis
Y.W. Kong, T.L. Willmott, R. Hu, K. Spriggs, S.A. Mitchell, A.E. Willis and M. Bushell
0383 Structural characterization of human ribosomal protein P2 by NMR spectroscopy
K.M. Lee, S.B. Chan, L.O. Chu, P.C. Shaw and K.B. Wong
0384 Translational microarray analysis of chronic lymphocytic leukaemia
L. Hornsby, H. Rogers, A. Willis, M. Dyer and T. Gant
0385 Identification and characterisation of mRNAs translated following DNA damage
I. Powley, M. Bushell, M. Stoneley and A.E. Willis
0386 Profiling non-Hodgkin’s lymphoma cell lines using translation cDNA microarray
P.J. Goodrem, M. Bushell and A.E. Willis
0387 Functional analysis of the insulin receptor IRES
S. Mitchell, K. Spriggs, S. Ridley, K. Siddle and A. Willis
0388 Further analysis of the BAG-1 IRES and specific trans-acting factors
H.C. Dobbyn, K.A. Spriggs, B.M. Pickering, J. Emsley and A.E. Willis
0389 The importance of scanning distance in N-myc IRES function
S. Haines, K. Spriggs, S. Mitchell, L. Cobbold and A. Willis
0390 Translational recruitment of pro-apoptotic genes via IRES elements during the induction of apoptosis
T. Willmott, I. Powley, A. Willis and M. Bushell

Lipid-protein interactions: mechanistic and functional studies

Abstracts 0391–0398, 0573–0574
Monday 18 July 18:00–19:30
0391 A study on the reconstitution of a G protein-coupled receptor into lipid vesicles
A. Robson, E. Compton and P. Booth
0392 Effects of lipids on the structure of the mechanosensitive channel of large conductance MscL.
J. Carney and A.G. Lee
0393 Human group IIA secreted phospholipase A2 (HgIIA); investigating membrane binding and its effect on host cell function
C.N. Birts and D.C. Wilton
0394 Spectroscopic studies of phospholamban variants in phospholipid bilayers
J.C. Clayton, E. Hughes, D.A. Middleton
0395 A genome-wide screen reveals a role of mitochondria in anaerobic uptake of sterols in yeast
S. Reiner, D. Micolod, G. Zellnig and R. Schneiter
0396 Functional and structural measurements of Ca21ATPase regulating transmembrane proteins
E. Hughes and D.A. Middleton
0397 Quantification of the effects of melittin on liposomes
J. Popplewell, N. Freeman, S. Carrington, G. Ronan, C. McDonnell and R. Ford
0398 Molecular dynamics simulations of spontaneous membrane protein micelle formation
P. Bond and M.S.P. Sansom

Systematic functional analyses of genomes

Abstracts 0399–0409, 0575–0579
Monday 18 July 18:00–19:30
0399 Functional mapping of toll/interleukin-1 signalling networks by transcription expression cloning
E. Kiss-Toth, D.H. Wyllie, K. Holland, L. Marsden, V. Jozsa, K.M. Oxley, T. Polgar, E.E. Qwarnstrom and S.K. Dower
0401 Exposure to low-dose X-rays promotes peculiar autophagic cell death in Drosophila melanogaster during metamorphosis, an effect that can be regulated by the inducible expression of E93 dsRNA
T. Kanao and Y. Miyachi
0402 Protein patterning on silicon (100)
C.W.A. Johnson, H. Yin, M.G. Gore and T. Melvin
0403 The rat model of estrogen-induced cholestasis: a pharmacogenomic study
L. Fernández-Pérez, L. Henríquez-Hernández, A. Flores-Morales, R. Santana-Farré, M. Axelsson and G. Norstedt
0404 Validation of metabolic flux balance model via synthetic lethal screening
R. Harrison, B. Papp, C. Pal, S. Oliver and D. Delneri
0405 Characterisation of neuro-active polypeptides from Aglena Orientalis
Z. Pan, A. Lipkin, E. Grishin and M. Soloviev
0406 A strategy for generating precise humanized mouse models using site-specific recombinases
D.A. Sorrell, C.B. Whitelaw, J. Ortego, L. Enjuanes and A. Kolb
0407 Screening platform for early immunoregulatory molecules in T cell receptor (TCR)-mediated signalling
C. Paar, W. Paster, M. Sonnleitner, B. Heise, S. Howorka, E.P. Klement, H. Stockinger and A. Sonnleitner
0408 Proteome analysis of Cryptosporidium
A.Z. Siddiki and J.M. Wastling
0409 Photonic generation of DNA micropatterns for haplotype analysis
H.B. Yin, T. Brown, J.S. Wilkinson, R.W. Eason and T. Melvin

Emerging cellular roles for actin polymerization

Abstracts 0410–0415, 0580
Monday 18 July 18:00–19:30
0410 Expression of connective tissue growth factor (CTGF) is sensitive to changes of the actin cytoskeleton
S. Muehlich, B. Krueger and M. Goppelt-Struebe
0411 Actin cytoskeleton and trafficking: the SH3-containing proteins role in yeast
G. Mirey, A. Soulard, C. Orange, S. Friant and B. Winsor
0412 Rho/ ROCK/ LIMK2 link TGF-b signalling to the actin cytoskeleton and control cell motility in Swiss3T3 fibroblasts
L. Vardouli, A. Moustakas and C. Stournaras
0413 Membrane androgen receptor activation regulates actin cytoskeleton reorganization in DU145 human prostate cancer cells
N.D. Papadopoulou and C. Stournaras
0414 Investigating dystroglycan deficiency at the cellular level – a role in cytokinesis?
J.R. Higginson and S.J. Winder
0415 Mapping the interactions of Scp1 with the actin cytoskeleton of Saccharomyces cerevisiae
D. Gheorghe, S. Winder and K. Ayscough

Topoisomerases and helicases: molecular motors on DNA

Abstracts 0416–0424
Tuesday 19 July 18:00–19:30
0416 Hel308/Mus308 helicase from archaea targets stalled forks in vivo and in vitro
E.L. Bolt and C.P. Guy
0417 Role of bipolar DNA motors in the RecBCD helicase/nuclease
M. Dillingham, M. Spies, M. Webb and S. Kowalczykowski
0418 On the conserved molecular architecture of type IIA topoisomerase
L. Farh, C.-W. Chiang, W.M. Huang, C.-C. Lin, C.-C. Hsu, H.-M. Lin, I.-P. Bi and N.-L. Chan
0419 Genome duplication in a complex protein rich environment
B. Payne, I. van Knippenberg and P. McGlynn
0420 DNA capture by the 43kDa ATPase domain of topoisomerase IV ParE
J.E. Riley, D.M.N. Parry and A.D. Bates
0421 Efficient blockage of DNA replication in vitro by protein-DNA complexes
I. van Knippenberg and P. McGlynn
0422 Targeting of PriA to stalled replication forks
P. Moon and P. McGlynn
0423 Progression of the E. coli replicative helicase on protein-bound DNA
J. Atkinson, B. Payne, I. van Knippenberg and P. McGlynn
0424 A model for DNA-stimulated ATPase activity on topoisomerase IV
D.M.N. Parry and A.D. Bates

New frontiers of cyclic AMP signalling

Abstracts 0425–0436, 0581
Tuesday 19 July 18:00–19:30
0425 Interaction of calmodulin with adenylyl cyclase type 8
R.E. Simpson and D.M.F. Cooper
0426 Role of Ca21 oscillations in regulating adenylyl cyclase type 8
A.C.L. Martin and D.M.F. Cooper
0427 Microtubule associate proteins (MAPs) regulate cyclic AMP signalling through exchange protein activated by cyclic AMP (EPAC)
S.J. Yarwood
0428 Critical role for ecto-59-nucleotidase (CD73) in vascular permeability during hypoxia
J.C. Morote, J.C. Ibla, H.K. Eltzschig, R. Resta, S.P. Colgan and L.F. Thompson
0429 Progesterone signalling via G proteins in filamentous fungus Rhizopus nigricans
N. Jeraj and H. Lenasi
0430 Comparisons of two calcium stimulated adenylyl cyclases, ACI and ACVIII
N. Masada, A. Ciruela and D.M.F. Cooper
0431 Drug discovery in Alzheimer’s using cyclic nucleotide focused gene array
I. McPhee, L.C.D. Gibson, J. Kewney, F. MacKenzie, C. Darroch, P. Stevens, C. Breslin, M. McFarlane and S.J. MacKenzie
0432 A molecular level model for the allosteric mechanism of cAMP from free RIIb PKA simulations
S. Pantano and M. Zaccolo
0433 Constitutive suppression of the nuclear accumulation of NF-kB in endothelial cells by A2A adenosine receptor gene transfer
E.W. Strong, W.A. Sands and T.M. Palmer
0434 Exchange protein activated by cAMP (EPAC)-mediated "suppressor of cytokine signalling-3" (SOCS-3) induction: a novel cAMP-triggered anti-inflammatory signalling pathway
W.A. Sands, H. Woolson, G.R. Milne and T.M. Palmer
0435 Priming of STAT1 and STAT3 for cytokine-mediated degradation upon A2A adenosine receptor (A2AAR) expression
M. Safhi, W.A. Sands and T.M. Palmer
0436 A novel assay platform for measuring cAMP
A. Fowler, S. Kumar, S. Zhao, C. Meck, Y.J. Choi, K. Peng, T. Naqvi, R. Singh, L. Kauffman and R. Eglen

Mechanisms of gene regulation

Abstracts 0437–0447, 0582–0585, 0607
Tuesday 19 July 18:00–19:30
0437 Expression screening identifies SM22 as a novel repressor of 92 kDa type IV collagenase expression
R. Nair and D. Boyd
0438 Epigallocatechin-induced haem oxygenase-1 expression is mediated by protein kinased in THP-1 monocytic cells
R.M. Ogborne, S.A. Rushworth and M.A. O’Connell
0439 Purification and characterization of M.SPRI multispecific DNA methyltransferase cloned and overexpressed in E. coli
A.A. Al-Ghanim, Q. Sheikh and D. Hornby
0440 Nrf2 and NF-kB mediate LPS-induced NAD(P)H: quinone oxidoreductase gene expression in human monocytic cells
S.A. Rushworth and M.A. O’Connell
0441 Activation in vitro of the CRK3 cyclin-dependent kinase of Leishmania mexicana
F.C. Gomes, K.M. Grant and J.C. Mottram
0442 Contribution of Src-FAK signalling to CTGF induction is modulated by cell-matrix interactions
A. Graness, I. Cicha and M. Goppelt-Struebe
0443 Antitumor activity of two DNA vaccines to inhibit tumor metastasis and growth
C.G. Park, Y.C. Yoo, S.Y. Chung, H.Y. Lee
0444 Atypical L-type channels are downregulated in hypoxia
L. Callinan, T.V. McCarthy, Y. Maulet and J.J. Mackrill
0445 PGC-1 a/b over-expression induces MHC fibre type changes and enhances citrate synthase activity and glycogen build-up in primary cultures of rat skeletal muscle
O.H. Mortensen, P. Schjerling, L. Frandsen, E. Nishimura and N. Grunnet
0447 Effects of reducing wild-type p53 gene dosage
C. Lynch, C. Rubbi and J. Milner

Biochemistry and molecular biology of variable antigenic surface proteins of parasites

Abstracts 0448–0454, 0586–0587, 0606
Wednesday 20 July 15:30–16:30
0448 Preferential hierarchies of VSG switching in T. brucei: analysis of antigenic variation using RNAi instead of an immune system
N. Aitcheson, S. Talbot, J. Shapiro, K. Hughes, C. Adkin, T. Butt, K. Sheader and G. Rudenko
0449 Studies on the chagasin family of cysteine peptidases inhibitors
C.C. Santos, F.C.G. Reis, T.F.R. Costa, A.P.C. Lima, G.H. Coombs and J.C. Mottram
0450 The role of protein enterotoxin producing bacteria in human diarrheal diseases
N. Amirmozafari
0451 Antigenic variation and transmission competence in Trypanosoma brucei
K. Matthews and F. van Deursen
0452 The myo-inositol-1-phosphate synthase gene is essential in Trypanosoma brucei
K.L. Martin and T.K. Smith
0453 Forward genetics isolates procyclic Trypansoma brucei cells defective in procyclin expression and GPI anchor modification
S.Y. Lee, A. Browitt, L. Tetley, J.D. Barry and A. Acosta-Serrano
0454 Dressing for the occasion: a new surface coat for Trypanosoma brucei in the salivary glands of the tsetse fly
S. Urwyler, A. Hemphill, C. Kunz Renggli, H. Imboden and I. Roditi

Obesity, appetite and adipokines

Abstracts 0455–0463, 0588
Wednesday 20 July 15:30–16:30
0455 Analysis of orexin signal transduction mediated by orexin receptor 1
G. Bart, H. Peltonen, J. Näsman, K. Larsson, J. Magga, L. Louhivuori and K. Åkerman
0456 The identification of leptin receptor in canine cruciate ligament cells and the influence of adipokines TNF alpha and leptin on matrix metalloprotease expression
V. Anderson, J.F. Innes and A. Vaughan-Thomas
0457 High throughput mutation scanning for MC4R by meltMADGE
K.K. Alharbi and I.N. Day
0458 GSK-3 inhibitors, but not insulin, stimulate glycogen synthesis in hepatocytes at low glucose concentrations
D. Schmoll, A. Riesenberg, D. Baum, M. Meyer and G. Müller
0459 Fatty acid modulation of B-lymphocyte gene expression, function and viability
R. Verlengia, R. Curi, R. Gorjão, T.M. de Lima and P. Newsholme
0460 LPS and cytokine induced changes in metabolism in the pancreatic b-cell
A. Kiely, S. Deveney and P. Newsholme
0461 Morphological and molecular analysis of adipose tissue in MAC16 tumour-induced cachexia
C. Bing, S. Russell, E. Becket, M.J. Tisdale and J.R. Jenkins
0462 Glutamine is required for 3T3 L1 cell proliferation, differentiation and lipid accumulation
M. Watford and Y. Wang
0463 Expression of interleukin-18 (IL-18) in human adipocytes: upregulation by TNFa
I.S. Wood, B. Wang, J.R. Jenkins and P. Trayhurn

Pfizer Research Colloquium - Free radicals and cyclic nucleotide signalling pathways: nitric oxide, reactive oxygen species, cAMP and cGMP

Abstracts 0464–0465, 0589
Wednesday 20 July 15:30–16:30
0464 Differentiation of osteoblast-like MC3T3-E1 cells increases sensitivity to oxidative stress damage
A.A. Fatokun, T.W. Stone and R.A. Smith
0465 ROS production after Zn effect in gill cells of Mytilus galloprovincialis
N. Evangelinos, G. Koliakos and M. Kaloyianni

Molecular aspects of protein translocation

Abstracts 0466–0470, 0590–0591
Wednesday 20 July 15:30–16:30
0466 Expression of WW domain-containing oxidoreductase in developing hearts and cardiac infarction
S.T. Chen, C.P. Lo, C.Y. Hong, Y.C. Lin and M.Y. Lee
0467 Structural and functional investigation of the protein translocation reaction driven by SecA through SecYEG
V. Gold
0468 Ser/Thr protein phosphatases constitutively associated with large-conductance calcium-activated potassium (BK) channels
G.A. Yazdi, M.J. Shipston and F. Antoni
0469 Novel homologues to the collagen-like protein SclC in Streptococcus equi are recognized by antibodies against SclC
Å. Karlström, K. Jacobsson and B. Guss
0470 Pro/Para: acid surfaces adapting on request. From transcription and chromatin remodelling to centrosomes, apoptosis and signal transduction
M. Frangou-Lazaridis and K. Vareli

Nitric oxide signalling - new targets, new biochemistry

Abstracts 0471–0474, 0592–0593
Wednesday 20 July 15:30–16:30
0471 Synergy between NO and superoxide in induction of cell death
V. Borutaite, H. Hope and G. Brown
0473 Weaning induces iNOS expression through NF-kB modulation in the lactating mammary gland. Importance of GSH
R. Zaragoza, V. Miralles, C. García, D. Rus, R. Carmena, E.R. García-Trevijano, T. Barber, F.V. Pallardó, L. Torres and J.R. Viña
0474 Nitric oxide release and extracellular matrix turnover induced by recombinant human erythropoietin in a porcine nasal cartilage explant model of rheumatoid arthritis
S. Baig, C. Fanutti, P. Coussons and R. Grant

Role of thiol redox changes in proteins involved in signalling and oxidative damage

Abstracts 0475–0480
Wednesday 20 July 15:30–16:30
0475 Selenoenzymes in the redox regulation
A. Kyriakopoulos and D. Behne
0476 Identification of potential acid/base catalysts in Plasmodium falciparum thioredoxin reductase
P.J. McMillan, L.D. Arscott, D.P. Ballou, C.H. Williams, Jr and S. Müller
0477 Characterisation of a testis-specific protein disulfide isomerase, PDILT
M. van Lith, D. Bown, J. Gatehouse and A. Benham
0478 Novel 1,4 benzoquinones as redox enzyme inhibitors
J.A. Irwin, C. Murray, M. Griffin, S. Brusey, K. Murnaghan, L. Thompson, M. Gallagher and G.G. Morgan
0479 Complex formation and tissue specific expression of the oxidoreductase Ero1L-beta
S. Dias Gunasekara, M. Van Lith, C. Dunne, D. Scoones, R. Kataky, J.A.G. Williams and A.M. Benham
0480 Identification of proximal protein targets of hydrogen peroxide induced signalling in mitochondria
T.R. Hurd and M.P. Murphy

Amyloidogenic proteins and their therapeutic implications

Abstracts 0481–0492, 0594
Wednesday 20 July 15:30–16:30
0481 The role of PrP’s N-glycans in the protein’s cellular location and biochemistry
F. Wiseman, E. Cancellotti, N. Tuzi, H. Baybutt, L. Aitchison, P. Monaghan, J. Ironside and J. Manson
0482 How SAFs are taken up and internalized by primary cultured neurons
C. Parkyn, R. Morris, A. Jen, R. Mootoosamy, E. Vermeulen and S. Standring
0483 Electrostatic contribution of reduced heat capacity change of unfolding in thermophilic L30e
C.F. Lee and K.B. Wong
0484 Crystal structure of a hyperthermophilic archaeal acylphosphatase from Pyrococcus horikoshii - structural insights into enzymatic catalysis, thermostability, and dimerization
S.Y. Lam, Y.-Y. Cheung, W.-K. Chu, M.D. Allen, M. Bycroft and K.-B. Wong
0485 Structural studies on fibrils formed from an a-synuclein peptide fragment
J. Madine, A.J. Doig and D.A. Middleton
0486 Functional characterisation of the N-terminal fragments resulting from two different proteolytic cleavages of the prion protein, PrP
Y.-H. Say and N. Hooper
0487 Novel HS analogues: inhibition of beta-secretase cleavage of amyloid precursor protein
S.J. Patey, C.J. Talbot, E.A. Edwards, E.A. Yates and J.E. Turnbull
0488 Inhibiting the palmitoylation and farnesylation of b-secretase reduces the production of amyloid-b, the protein involved in the development of Alzheimer’s disease
R.B. Parsons and B.M. Austen
0489* Solution structure of proteins from raman optical activity
F. Zhu, N.W. Isaacs, L. Hecht and L.D. Barron
0490 Low dose statins reduce Ab production by inhibiting palmitoylation – a mechanism independent of cholesterol
R.B. Parsons, J.K. Farrant, G.C. Price and B.M. Austen
0491 b-secretase activity in platelets from Alzheimer’s disease and control subjects
W.W. Liu, S.A. Todd, D.T.R. Coulson, S. Murphy, G.B. Irvine, A.P. Passmore and J.A. Johnston
0492 Reactive oxygen species (ROS)-mediated b-cleavage of the prion protein in the mechanism of the cellular response to oxidative stress
N.T. Watt, D.R. Taylor, A. Gillott, D.A. Thomas, W.S.S. Perera and N.M. Hooper
*Poster 0489 will be presented on Tuesday 19 July 18:00–19:30

Developmental mechanisms

Abstracts 0493–0508
Monday 18 July, 18:00–19:30
0493 Transdifferentiated hepatocytes: an in vitro model for liver function
K.L. Ralphs, Z.D. Burke, C.N. Shen and D. Tosh
0494 Isolation and characterization of histidine kinase Le.nik1 in Lentinula edodes related to stress response and development.
Y.Y. Szeto, W.L. Wong and H.S. Kwan
0495 Studies on somatotropin (growth hormone) isolated from local species of water buffalo (Bubalus bubalis) from Pakistan
A.J. Sami and S. Ali
0496 The role of cerebrospinal fluid on neural cell survival in the developing chick cerebral cortex: an in vivo study
Z. Salehi and F. Mashayekhi
0497 High throughput mutation scanning for LDLR by meltMADGE
K.K. Alharbi and I.N. Day
0498 Nerve growth factor regulates the death of neurons in developing chick cerebral cortex: an in vivo study
F. Mashayekhi and Z. Salehi
0499 Using of antisense oligonucleotides for modulation of gene expression in early human embryo.
E. Zakharova, M. Semenova, N. Kosheleva, V. Zaeva and S. Zaletov
0500 Proteomic analysis of in vivo phosphorylation of juvenile MAP1B; adult and phylogenetic comparisons
S. Lin, R.G. Goold, J. Dunlop and P.R. Gordon-Weeks
0501 The 59-39 exoribonuclease pacman and its role in wound healing
M.J. Sullivan, M.V. Zabolotskaya, H.L. Glenwright and S.F. Newbury
0502 Ciliary and cement gland cells on the surface of anuran embryos and larvae: from pattern and structure to function
M. Nokhbatolfoghahai and R. Downie
0503 Poster withdrawn
0504 The role of pacman protein in adult Drosophila male fertility
M.V. Zabolotskaya, C.L. Roberts and S.F. Newbury
0505 Insulin signalling regulates mitochondrial function and oxidative phosphorylation protein content in the murine heart
S. Boudina, S. Sena, B.T. O’Neill, V. Zaha, P. Mazumder, J. Wright, H. Theobald, I. Rasmussen and E.D. Abel
0506 C/EBPalpha and C/EBPbeta expression during mouse embryonic development – possibilities for a developmental switch between pancreas and liver?
A. Westmacott, Z.D. Burke, J.M.W. Slack and D. Tosh
0507 Inhibition of cell migration by PTEN and TPTE
N.R. Leslie, X. Yang, C.P. Downes and C.J. Weijer
0508 Pico: a novel regulator of insulin receptor signalling
E. Lyulcheva and D. Bennett

Making biosciences teaching FUN!

Abstract 0511
Monday 18 July 18:00–19:30
0511 Evaluate a rasmol-based assessment instrument for facilitating protein structure learning
S.-J. Lee and L. Farh

Mechanisms of ABC transporters

Abstracts 0512–0517, 0595–0598
Wednesday 20 July 15:30–16:30
0512 Preliminary single particle analysis of P-glycoprotein reveals dimeric organisation
A.B. Kamis, R. Callaghan, J. Storm and R.C. Ford
0513 Crystallographic and single particle analyses of native and nucleotide-bound forms CFTRprotein
N.H. Awayn, M.F. Rosenberg, L.A. Aleksandrov, J.R. Riordan and R.C. Ford
0514 The role of non-transporting ABC proteins in antibiotic resistance
H. Jeffery, P. Ellis, W. Grainger and I.D. Kerr
0515 Helical peptide found in the channel of the outer membrane protein TolC
C. Fanutti, P.J. Coussons and A. Hemmings
0516 Flowcytometry allows accurate determination of anthracycline intracellular compartmentalization and various ABC-transporters’ function in multidrug resistant cells
A. Ravcheeva, A. Konukhova, A. Marieva, N. Vichljantzeva, T. Zabotina and T. Bogush
0517 The membrane protein structure initiative (MPSI): a glasgow perspective
K. McLuskey, I. Black, A.W. Roszak, D. Hadden, A. Ward, S.E. Palmer, S.A. Baldwin, P.J.F. Henderson, R.J. Cogdell and N.W. Isaacs

Regulation of signalling by cellular location and sequestration

Abstracts 0518–0540, 0599–0600
Wednesday 20 July 15:30–16:30
0518 Arginine-vasopressin activates the JAK-STAT pathway in vascular smooth muscle cells
L. Oren and G. Yossef
0519 Spermatogenesis under stress: interchanging AChE-R - RACK1/Enolase-a interaction modulate sperm development and metabolism
I. Mor, E.H. Sklan, M. Pick, M. Kirschner, L. Yogev, S. Diamant, E. Podoly, S. Fletscher, T. Mor, L. Schreiber, D. Grisaru and H. Soreq
0520 Mutation of phosphorylation sites within phospholipase D affects its interaction with actin
M.J. Farquhar, M.H. Wright, D.J. Powner, B.A. Levine, G. Ladds and M.N. Hodgkin
0521 Biosynthesis and trafficking of seven transmembrane receptor signalling complexes
D.J. Dupré, A. Baragli, N. Éthier, L. Villeneuve, T.E. Hébert
0522 The GTP-binding protein Rap1A is hypoprenylated during statin-induced muscle toxicity in the rat
P.D. Glaves, K. Lowry, F.W. Westwood, A.M. Marsden, R.C. Scott and J.E. Sidaway
0523 Antagonist pre-incubation differentiates compounds in a FLIPR-based ligand-gated ion channel assay
C.L. Leech, G.R. Chan, D. Johnson, K.G. Sutton, P.B. Simpson and G. Richards
0524 Multiple assay formats for measuring functional responses at stably expressed human vasopressin V1b receptors
M.S. Ainger, E. Garrett, C. Nanthakumar, A.R. Rutter, J. Kerby, P.B. Simpson and A.J. Smith
0525 Desensitisation of the calcitonin-like receptor in the human neuroblastoma SK-N-MC cell line
K.M.E. Chu and A.J.L. Clark
0526 PGF-FP receptor regulation of fibroblast growth factor 2 (FGF2) expression
K.J. Sales, A.R.W. Williams, R.A. Anderson and H.N. Jabbour
0527 Mitochondrial ion flux in cell death signalling in platelets
H.A. Leaver and C.V. Prowse
0528 CD38 associated with lipid rafts is an active ADP-ribosyl cyclase
E. Zumaquero, G. Lucena, P. Muñoz, E. Pavón, D. Herrero, A. Martín, D. González-Pacanowska, L.-M. Ruiz Pérez, F. Malavasi, J. Sancho and M. Zubiaur
0529 Caveolin-1 down-regulates expression of survivin via inhibition of b-catenin-Tcf/Lef dependent transcription
V.A. Torres, J. Tapia, L. Leyton and A.F.G. Quest
0530 GnRH-induced activation of Jak/STAT signalling
A.J. Pawson, S. Gardner, S. Maudsley, L. Davidson, R. López de Maturana and R.P. Millar
0531 GnRH-induced inhibition of glycogen synthase kinase-3b and activation of Wnt/b-catenin signalling pathways
S. Gardner, S. Maudsley, A.A. Thomson, R.P. Millar and A.J. Pawson
0532 Ligand-induced selective signalling (LiSS) at the gonadotropin-releasing hormone (GnRH) receptor
J. Hart, A.J. Pawson, R. López de Maturana, Z.-L. Lu, K. Morgan, S. Maudsley, L. Davidson, Z. Naor and R.P. Millar
0533 A novel inside-out prostanoid signalling pathway that mediates GnRH receptor auto-regulation
Z. Naor, H.N. Jabbour, M. Naidich, A. Pawson, K. Morgan, S. Battersby, M. Millar, P. Brown and R.P. Millar
0534 Structural and functional characterisation of the interaction of the dihydropyridine receptor II-III loop with the ryanodine receptor
M.G. Casarotto, Y. Cui, Y. Karunasekara, P.J. Harvey, N. Norris, P.G. Board and A.F. Dulhunty
0535 Differential regulation of the nuclear localisation of GRK4 subfamily members
L.R. Johnson and J.A. Pitcher
0536 Evidence for GnRH ligand-selective receptor active conformations
Z.-L. Lu, R. Gallagher, N. Miller, V. Warrender, R. Sellar and R. Millar
0537 HitHunter kinase assays: a comprehensive and homogeneous screening platform
A. Fowler, S. Kumar, L. Kauffman, U. Nguyen, H. Dotimas, S. De La Llera, V. Shenoy, D. Pao, R. Singh and R. Eglen
0538 ADP Quest: a novel homogeneous, generic, kinase for kinase assay
A. Fowler, S. Kumar, N.W. Charter, V. Shenoy, U. Nguyen, L. Kauffman and R. Eglen
0539 Two functionally distinct pools of PDGF-activated Src kinases in fibroblasts.
L. Veracini, M. Franco, A. Boureux, V. Simon, S. Roche and C. Benistant
0540 Ryanodine receptor regulation of smooth muscle matrix metalloprotease-2
A. Corr, T.V. McCarthy, V. Sorrentino and J.J. Mackrill

Miscellaneous

Abstracts 0541–0555, 0601–0604
Tuesday 19 July 18:00–19:30
0541 The contribution of p47-phox phosphorylation to leukocyte priming mechanisms associated with pre-eclampsia.
T.M. Poolman, L. Solyakov, A.B. Tobin and L.L. Ng
0542 Characterization of the inhibition of xanthine oxidase by flavonoids and tannins from two medicinal plants: Fraxinus angustifolia and Pistacia lentiscus
K. Ayouni, M. Berboucha, Dina Atmani and Djebbar Atmani
0543 Production and purification of rabbit polyclonal antibody against human T-lymphocytes (ATG)
H. Javanmard, J. Majidi, R. Estakhri and M.B. Abdi
0544 The role of connexins in the coordination of cell growth
S. Johnstone, R.J. Errington and P.E.M. Martin
0545 Detection of early-stage protein crystallisation by dual polarisation interferometry
N. Freeman, A. Boudjemline and S. Carrington
0546 Measurement of conformational change in MAP kinase on drug candidate binding
M. Gostock, K. Chapman, G. Ronan and S. Carrington
0547 Interaction of WR99210 with dihydrofolate reductase thymidylate synthase (DHFR-TS) from Plasmodium vivax
P.K. Aitha, M. Malhotra, A.K. Misra, V. Kothekar and T. Nandi
0548 Calcium-independent phospholipase A and agonist-induced calcium entry
K. Ross and N.J. Reynolds
0549 Functional expression of human sodium/D-glucose cotransporter1
N.K. Tyagi, A. Kumar and R.K.H. Kinne
0550 Development of an ataxin-3-based screening assay for the identification of potentially antiamyloidogenic compounds
M. Riva, S. Gobbo, M. Valtorta, P. Tortora and P. Fusi
0551 Peptidomimics as ACE inhibitors: a new approach to longer bioavailability
K. Hanif, M.C. Pavar, M.A.Q. Pasha and S. Pasha
0552 Reducing crystallization failures earlier by using microgram quantities of protein for crystallization trials with TOPAZ microfluidics
R. Lussier
0554 Molecular aspects of hepatitis C virus (HCV) in patients on chemotherapy
N. Ahmad, M. Asghar, A. Tariq, M. Shafiq and J.A. Qureshi
0555 Protein fingerprinting technique for diagnosis of diabetes mellitus
A. Sharif, N. Ahmad, A. Hameed, F. Rabbi, W. Hussain, M. Asghar, M.T. Iqbal and J.A. Qureshi

Late breaking abstracts

The keys to actin: regulators of the Arp2/3 complex

Abstract 0556
Monday 18 July 18:00–19:30
0556 Actin nucleators in plants
M.J. Deeks, D. Kaloriti, L.M. Machesky, R. Malhó, B. Davies and P.J. Hussey

Mechanisms of crossing-over: genetic and biochemical approaches

Abstract 0557
Tuesday 19 July 18:00–19:30
0557 Sgs1p prevents inappropriate meiotic recombination in S. cerevisiae
A. Chaix and R.H. Borts

ARF GTPases: integrators of lipid signalling, membrane traffic and actin

Abstracts 0558–0561
Tuesday 19 July 18:00–19:30
0558 Interactions between Sla1p, Lsb5p and Arf3p in yeast endocytosis
R. Costa and K.R. Ayscough
0559 Centaurin-a1, an ADP-ribosylation factor 6 GTPase activating protein, inhibits b2-adrenoceptor internalization
J. Lawrence, S.J. Mundell, H. Yun, E. Kelly and K. Venkateswarlu
0560 Centaurin-a1 interacts directly with kinesin motor protein KIF13B
K. Venkateswarlu, T. Hanada and A.H. Chishti
0561 Biochemical and cellular analysis of ARF6 like protein
H. Yun and K. Venkateswarlu

Stem cells in development and disease

Abstract 0562
Tuesday 19 July 18:00–19:30
0562 Stage- and site-specific DNA demethylation during neural cell development from embryonic stem cells
K. Shimozaki, M. Namihira, K. Nakashima and T. Taga

Molecular clocks (2): conservation and convergence of clock elements

Abstract 0563
Tuesday 19 July 18:00–19:30
0563 An influence of melanopsin on the cone visual pathway in mice
A.R. Barnard, S. Hattar, M.W. Hankins and R.J. Lucas

Metabolomics: the link between genomics, proteomics and phenotype

Abstracts 0564–0565
Tuesday 19 July 18:00–19:30
0564 Effects of polycyclic aromatic hydrocarbons on plasma protein expression and DNA damage in mice
D.G. Sul, W.W. Jung and C.K. Lee
0565 Proteomic analysis of Arabidopsis cell nuclei
M. Kotlinski, J. Sikora, J. Oledzki, T. Calikowski, M. Dadlez and A. Jerzmanowski

Chromatin in development and disease

Abstracts 0566–0567
Wednesday 20 July 15:30–16:30
0566 The Swi2/Snf2 bromodomain is required for the remodelling activity of SWI/SNF on acetylated nucleosomes
P. Prochasson, J.L. Workman and A.H. Hassan
0567 Poster withdrawn

Mechanisms of synapse assembly and plasticity

Abstract 0568
Wednesday 20 July 15:30–16:30
0568 Mitochondria in dendrotoxicity
S.M. Greenwood and C.N. Connolly

Translational control in development

Abstracts 0569–0570, 0605
Wednesday 20 July 15:30–16:30
0569 Bruno acts as a bifunctional repressor of oskar mRNA translation
M. Chekulaeva, M. Hentze and A. Ephrussi
0570 Enhanced protein synthesis activity during the late growth phase in an antibiotic overproducing rpsL mutant of Streptomyces coelicolor A3(2)
T. Hosaka, J. Xu and K. Ochi

Mechanisms of translation

Abstracts 0571–0572
Wednesday 20 July 15:30–16:30
0571 Functional and structural analysis of a new class of picornavirus internal ribosome entry site (IRES)
L.S. Chard, Y. Kaku and G.J. Belsham
0572 A microarray-based approach to reveal the extent of PTB-dependent translation initiation within mammalian cells
M. Stoneley, K.A. Spriggs, M. Bushell and A.E. Willis

Lipid-protein interactions: mechanistic and functional studies

Abstracts 0573–0574
Monday 18 July 18:00–19:30
0573 The rat luteinizing hormone receptor ectodomain splice variant is retained in the endoplasmic reticulum in human embryonic kidney 293 cells
P. Apaja, J. Tuusa, H. Rajaniemi and U. Petäjä-Repo
0574 Localization and Rab protein interaction profiles of YIP1 family members
C.Z. Chen and R.N. Collins

Systematic functional analyses of genomes

Abstracts 0575–0579
Monday 18 July 18:00–19:30
0575 A new high capacity nickel charged medium for batch purification of histidine-tagged proteins
M. Carlsson, A. Bergh, A. Heijbel, J. Lundqvist, U. Saplakoglu, K. Torstenson and K. Öberg
0576 HisTrap FF crude for faster purification of histidine-tagged proteins
A. Bergh, L.C. Andersson, E. Ancker, A. Heijbel, H. Lindgren, J. Lundqvist, K. Torstenson and K. Öberg
0577 HPV detection and genotyping using HPV DNA chip in cervical swabs
W.W. Jung, H.S. Kim, I.K. Han, D.J. Lee, C.K. Lee and S.G. Lee and K.J. Jo and S.K. Kim
0578 CamKinBase database for human calmodulin dependent protein kinases
T. Horváth, C. Ortutay and M. Vihinen
0579 Cell array-based subcellular localization of human chromosome 21 proteins
Y.-H. Hu, D. Vanhecke, H.-J. Warnatz, F. Wagner, A. Fiebitz, S. Thamm, M.-L. Yaspo, H. Lehrach and M. Janitz

Emerging cellular roles for actin polymerization

Abstract 0580
Monday 18 July 18:00–19:30
0580 Mechanisms of PKCe-induced neurite outgrowth
L. Sunesson

New frontiers of cyclic AMP signalling

Abstract 0581
Tuesday 19 July 18:00–19:30
0581 Crosstalk between cAMP signalling pathway and ERK pathway
A. Gormand, O. Sturm, G. Baillie, M. Houslay and W. Kolch

Mechanisms of gene regulation

Abstracts 0582–0585, 0607
Tuesday 19 July 18:00–19:30
0582 Interleukin-1 receptor antagonist gene polymorphism is associated with increased risk of primary biliary cirrhosis
H.A. Çelik, H.H. Aydin, M. Akyildiz, F. Günsar, Y. Batur and A. Berdeli
0583 A region of herpesvirus ICP27 homologue proteins involved in nucleolar localisation and nucleocytoplasmic shuttling
C. Leiper, E. Mertens, J. Kean, P. Malik and B. Clements
0584 Mechanisms of RNA polymerase III activation by c-myc
N.S. Kenneth and R.J. White
0585 c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I
N. Gomez-Roman, C. Grandori, Z.A. Felton-Edkins, C. Ngouenet, D.A. Galloway, R.N. Eisenman and R.J. White

Biochemistry and molecular biology of variable antigenic surface proteins of parasites

Abstracts 0586–0587, 0606
Wednesday 20 July 15:30–16:30
0586 Biophysical and biochemical studies on ComH, a unique H. pylori protein involved in DNA uptake
K. Kostidis, C.W. Potter, J.R. Sayers and C. Bardhan
0587 A mouse aldo-keto reductase confers resistance to toxic aldehydes and ketones in vitro
D. Li, R. Gardner, A. Hinshelwood and E. Ellis

Obesity, appetite and adipokines

Abstract 0588
Wednesday 20 July 15:30–16:30
0588 Elucidation of the mechanism of mitochondrial uncoupling protein function using a synthetic glycolipid (glucose-O-v-palmitate).
S.G. Gouin, E.P. Breen, W. Pilgrim, P.V. Murphy and R.K. Porter

Pfizer Research Colloquium - Free radicals and cyclic nucleotide signalling pathways: nitric oxide, reactive oxygen species, cAMP and cGMP

Abstract 0589
Wednesday 20 July 15:30–-16:30
0589 Flow cytometric kinetic assay of reactive oxygen species (ROS) in whole blood platelets: a simple assay to assess oxidative stress in blood cells
M. Fortuna, M. do Céu Monteiro, M.J. Gonçalves and J.E. O’Connor

Molecular aspects of protein translocation

Abstracts 0590–0591
Wednesday 20 July 15:30–16:30
0590 Electron-crystallography of a conserved mediator of membrane protein assembly
M. Lotz, W. Haase and I. Collinson
0591 Pathways for protein transport to seed storage vacuoles
N. Jolliffe, C. Craddock, J. Brown, L. Roberts and L. Frigerio

Nitric oxide signalling - new targets, new biochemistry

Abstracts 0592–0593
Wednesday 20 July 15:30–16:30
0592 Arginine uptake is attenuated, through modulation of cationic amino acid transporter-1, in uremic rats
I.F. Schwartz and D. Schwartz
0593 Poster withdrawn

Amyloidogenic proteins and their therapeutic implications

Abstract 0594
Wednesday 20 July 15:30–16:30
0594 Meptides: N-methylated peptide inhibitors of b-amyloid aggregation
H. Amijee, N. Kokkoni, A.J. Doig, K. Stott, J.M. Treherne and D.I.C. Scopes

Mechanisms of ABC transporters

Abstracts 0595–0598
Wednesday 20 July 15:30–16:30
0595 Gel electrophoresis and EM studies of the oligomeric state of ABC transporters
L. Zhang, S. Modok, N.H. Awayn, C. McDonnell, R.F. Collins, M.F. Rosenberg, R. Callaghan and R.C. Ford
0596 Structural and functional studies of P-glycoprotein
C. McDonnell, R.C. Ford and J. Popplewell
0597 Drug/free lipid A interactions in the Escherichia coli ABC-transporter MsbA
B. Woebking, G. Reuter, R.A. Shilling, S. Velamakanni, S. Shahi, H. Venter, L. Balakrishnan and H.W. van Veen
0598 Expression of membrane proteins for structural studies
M. Rahman, M.J. McPherson and S.A. Baldwin

Regulation of signalling by cellular location and sequestration

Abstracts 0599–0600
Wednesday 20 July 15:30–16:30
0599 A novel interaction between Krp1 and Lasp-1 is involved in pseudopodia formation in a Rho and ROCK independent manner
H.J. Spence, L. McGarry, L.A. Carragher, N. Carragher, C.S. Chew and B.W. Ozanne
0600 Metastasis-associated protein S100A4 as a modulator of random cell migration in the model of epithelial-mesenchymal transition
J. Mejlvang, E. Tulchinsky, J.K. Mellon and M. Kriajevska

Miscellaneous

Abstracts 0601–0604
Tuesday 19 July 18:00–19:30
0601 IP1 as a surrogate of IP3 to monitor Gq coupled GPCR response in a HTS format
E. Trinquet, M. Fink, F. Grillet, F. Maurin, E. Bourrier, H. Bazin, N. Gregor, S. Sulocha, A. Michaud, J.L. Tardieu, C. Goudet, F. Malhaire, J.P. Pin, T. Durroux, D. Maurel, J. Bernard and E. Burgeon
0602 Tryptophan 64 is a passive reporter for the conformational change in glutamate dehydrogenase from Clostridium symbiosum.
M.A. Hamza, S.R. Martin and P.C. Engel
0603 Post-translational regulation of Fos-related antigen-1 (Fra-1) in cancer cells.
R. Stanford, R. Vickery, R. Edwards, P. Greaves, A. Tobin, J.K. Mellon and E. Tulchinsky
0604 A novel approach to rrnD promoter and RNAP interaction using double strand DNA with surface plasmon resonance technology
H. Aoki and K. Ochi
0605 Evidence of translational regulation in human embryonic stem cells
I.A. Bouhon and R.A. Pedersen
Poster will be presented on Wednesday 20 July 15:30–16:30
0606 The products downstream of the mevalonate pathway are essential in Trypanosoma brucei
T.K. Smith, T. Chang, J.A. Nunes, K.L. Martin and G. Ricmond
Poster will be presented on Wednesday 20 July 15:30–16:30
0607 Expression and characterisation of viral DNA-binding proteins
B. Saran, K. Kostidis and J.R. Sayers
Poster will be presented on Tuesday 19 July 18:00–19:30